Production of intracellular biosurfactants for the production of biosurfactant ml of mineral salt medium and yeast. To produce biosurfactants from pseudomonas aeruginosa using agricultural resource and to produce biosurfactants using low cost materials. The results indicated that the specific growth rates. Production and characterization of biosurfactant from pseudomonas spp s. Pdf this study deals with production and characterization of biosurfactant from renewable resources by pseudomonas aeruginosa.
Applications of biosurfactant obtained from pseudomonas. A hydrocarbondegrading, biosurfactantproducing, and plantgrowthpromoting endophytic bacterium, pseudomonas aeruginosa l10, was. Comparative study for biosurfactant production by using. Wed like to understand how you use our websites in order to improve them. Salicylic acid affects swimming, twitching and swarming. Isolation and production of biosurfactant from pseudomonas. The strains started rhamnolipid production in the logarithmic phase. However, according to recent studies these products can be exploited for human benefits. The study was initiated with the cultivation of pseudomonas aeruginosa usmar2 in a bioreactor, followed by the simultaneous recovery of rhamnolipid. Effect of the carbon source on biosurfactant production by. Biosurfactant production by pseudomonas aeruginosa a41 using palm oil as carbon source. Only one isolate, pseudomonas aeruginosa ukmp14t, was found to be positive for all the qualitative. Glycolipid production by isolated bacterium using different carbon gasolin, paraffin oil, glycerol, whey and nitrogen sources nano3, nh42so4 and ch4n2o was studied. Structural and physicochemical characterization of a.
This microorganism was isolated from a soil artificially contaminated with crude oil and used to produce rhamnolipid using glycerol as the carbon source. Selection of pseudomonas aeruginosa for biosurfactant. Studies on production of biosurfactant from pseudomonas. Rhamnolipid production pseudomonas aeruginosa pa1 was inoculated in erlenmeyer flasks containing 600ml of culture medium. Use of waste canola oil as a lowcost substrate for. Screening of pseudomonas species for biosurfactant.
Biosurfactant production by pseudomonas aeruginosain kefir and. To produce biosurfactants from pseudomonas aeruginosa using agricultural resource and to produce biosurfactants using low. Rhamnolipid is a biosurfactant produced by pseudomonas aeruginosa and has a. Biosurfactants are surfactants of biological origin, produced by. The pseudomonas aeruginosa atcc 10145 strain and isolated pseudomonas aeruginosa were able to reduce the surface tension of the initial medium from 61 mnm to 32. Figure 1 ftir analysis of biosurfactant extracted from pseudomonas putida. This was followed by the addition of glycerol to test their influence on biosurfactant production. Application of rhamnolipid biosurfactant produced by.
Biosurfactant production was quantified by surface tension reduction, critical micelle dilution cmd. Biosurfactant production by pseudomonas aeruginosa strains. By considering the importance of biosurfactants and their role in biodegradation of hydrocarbons and other industrial applications, the present investigation was conducted with the following objectives. Pdf biosurfactant production by pseudomonas aeruginosa from. The present investigation demonstrates the utilization of biodieselderived crude glycerol as a lowcost substrate for the production of biosurfactant. Physicochemical properties of rhamnolipid biosurfactant. Application of biosurfactants produced by pseudomonas aeruginosa sp4 for bioremediation of soils contaminated by pyrene. On the basis of biomass production and biosurfactant production was the strain p. Biosurfactant production by pseudomonas aeruginosa dsvp20. It is found in soil, water, skin flora, and most manmade environments throughout the world and can easily be extracted from sea water sample for production of biosurfactants. Also, surface tension of a biosurfactant product remained. Production of biosurfactants from oil sludge using.
Biosurfactant production by pseudomonas aeruginosa in. Pseudomonas aeruginosa produces an extracellular glycolipid surfactant called rhamnolipids 4 and composed of. In this study isolation, screen and identification of pseudomonas bacteria from oil sludge, was capable producing biosurfactant to emulsify crude oil. The surface tension st reduction, biosurfactant production, and antifungal activity of biosurfactant against c. Pdf application of biosurfactants produced by pseudomonas. In this study, we explored the potential of waste canola oil as a lowcost and environmentally friendly substrate for the production of rhamnolipids by p. This study involves the screening of biosurfactant producers that have been isolated from crude oil bacteria degraders. Furthermore, the stability of the biosurfactant relative to some environmental stress conditions ph, temperature and salinity was also evaluated. Usharani department of microbiology, annamalai university, annamalai nagar608 002 tamil nadu, india abstract. The bacteria used for production of biosurfactants were selected, two different types of biosurfactants including lipopeptides, rhamnolipids produced by bacillus subtilis and pseudomonas aeruginosa, respectively. Surface tension was used as a preliminary screening standard for potential biosurfactant bacteria. Pseudomonas aeruginosa 44t1 produces rhamnolipids when grown on c12 nalkane but not with other hydrocarbons tested.
The methodology included the isolation of microorganisms, standardization of batch culture conditions for good surfactant production and characterization of the produced rhamnolipid. Biosurfactant production by pseudomonas aeruginosa a41, a strain isolated from seawater in the gulf of thailand, was examined when grown in defined medium containing 2% vegetable oil or fatty acid as a carbon source in the presence of vitamins, trace elements and 0. Use of soybean oil fry waste for economical biosurfactant. Improved biosurfactant production by a pseudomonas. Karadzic2 1faculty of chemistry, university of belgrade. Screening of media for the biosurfactant production. Biosurfactant surface activity is expressed as a reduction percentage of the surface tension reduction by the following. Coal flotation using a biosurfactant from pseudomonas. In this study, the biosurfactant produced by pseudomonas aeruginosa was evaluated in view of its ability to be used in microbialenhanced oil recovery meor. Characterization of partially purified biosurfactant by lcmsms analysis indicated predominant production 78% of dirhamnolipids rharhac10c10 and rharha. Biosurfactant production by pseudomonas aeruginosa snp0614. The ability of strain pseudomonas putida to grow and produce biosurfactant on different carbon sources, temperatures and ph was studied. By adhering to many different surfaces by means of virulence factors and motility, p. P1 strain, isolated from heavily petroleum hydrocarboncontaminated soil, produced biosurfactant and used in biore.
The bacteria were isolated by qualitative screening on cetyltrimethylammonium bromide ctab agar plates and quantitative screening for biosurfactant production in liquid media. Biosurfactant production by pseudomonas aeruginosa strains on 2ml of inoculum size 699 p j m h s vol. Rhamnolipids are glycolipid biosurfactants that are primarily produced by pseudomonas aeruginosa that have gained a great deal of interest for their numerous industrial applications and environmentally friendly properties. This strain was selected for optimization for biosurfactant production and its characterization. Rhamnolipid biosurfactants specifically produced by pseudomonas aeruginosa have great potential for industrial. Rhamnolipid purification was carried out by extraction, using a chloroformmethanolculture medium mi x. Biosurfactant production by pseudomonas aeruginosa. Standardized effects of the factors on the recovery percentage of combustible matter. This study deals with production and characterization of biosurfactant from renewable resources by pseudomonas aeruginosa. In silico engineering of pseudomonas metabolism reveals. Biosurfactants are amphiphilic compounds produced by various bacteria and fungi which reduce surface and interfacial. Pseudomonas aeruginosa, biosurfactant, hydrocarbons emulsification activity, surface tension in this present study, biosurfactant producing microorganism pseudomonas aeruginosa pbsc1, was isolated from mangrove ecosystem in pichavaram boat house, tamil nadu, india. Ds9 extracted from the bacterial strain pseudomonas aeruginosa ds9 was evaluated for its antifungal activity against colletotrichum falcatum that causes red rot in sugarcane.
Pseudomonas aeruginosa biosurfactant production in continuous. Get a printable copy pdf file of the complete article 939k, or click on a page. Gq865644 based on biochemical characterization and the 16s rdna analysis, and it was found to be a potential candidate for biosurfactant production. Studies on biosurfactant production by pseudomonas. The present study describes the structural characterization and biotechnological application of a dirhamnolipid biosurfactant produced by pseudomonas aeruginosa strain nbtu01 isolated from a petroleum oilcontaminated soil sample. Biosurfactant production was carried out in 3l fermentor using waste motor lubricant oil and peanut oil cake. Rhamnolipid is a biosurfactant produced by pseudomonas aeruginosa and has a strong potential to be used in industries and bioremediation rashedi et al. This bacterium was found to produce rhamnolipid, a glycolipidtype of biosurfactant. Comparative study for biosurfactant production by using bacillus subtilis and pseudomonas aeruginosa t. This resulted in a process with a combined rhamnolipid production and recovery. Characterization of biosurfactant produced by pseudomonas. The final step in rhamnolipid biosynthesis is catalyzed by the rhlab genes encoding a rhamnosyltransferase. Pseudomonas aeruginosa biosurfactant production in continuous culture with.
The bacterial strains such as saccharomyces cerevisiae and pseudomonas aeruginosa were isolated from the petroleum contaminated soil samples and they were screened by using oil spreading method youssef et al. Biosurfactant were produced from local bacteria and investigated production of biosurfactant at different source carbon. Production and characterization of biosurfactant from. Pseudomonas aeruginosa ds10129 produced a maximum of 1. Biosurfactant from pseudomonas aeruginosa pa1 to applications in microemulsions. Kinetics of biosurfactant production by pseudomonas.
The highlevel production of rhamnolipid biosurfactants is a unique feature of pseudomonas aeruginosa and is strictly regulated in response to environmental conditions. Rhamnolipid biosurfactant from pseudomonas aeruginosa. Screening and molecular analyses for biosurfactant production by pseudomonas aeruginosa strains from oil polluted soil received for publication, september, 23, 2016 accepted, march, 15, 2017 robertina ionescu1, ioana mereuta2, diana pelinescu1, ortansa csutak1, anamaria tanase1, tatiana vassu1, ileana stoica1. An external file that holds a picture, illustration, etc. Isolation and screening of biosurfactants produced by. The presence of biosurfactant was determined by the dropcollapse test, microplate analysis, oil spreading technique, emulsification index %ei 24 and surface tension measurement. Pdf biosurfactant production by pseudomonas aeruginosa.
Screening and molecular analyses for biosurfactant. Srinikethan department of chemical engineering, national institute of technology, surathkal, mangalore,n india. Screening and optimization of biosurfactant production by. Pseudomonas aeruginosa confirmed by persian type culture collection. Improved biosurfactant production by a pseudomonas aeruginosa strain using agroindustrial wastes eduardo j. Batch kinetic studies were carried out on rhamnolipid biosurfactant production from synthetic medium, industrial wastes viz. This study suggested that pseudomonas sps showed the maximum biosurfactant production. Optimization of biosurfactant production from pseudomonas. In the present work the effects of culture media components on yield of these two products from p. The biosurfactant production by strains of pseudomonas aeruginosa isolated from colombian hydrocarbon contaminated sludges has been determined. A biosurfactant producer identified as pseudomonas aeruginosa 181 was selected for further analysis. One of the main limitations for the biosurfactant production in bioreactor is the intense foaming formation when the solution is aerated and agitated caused by the surfactant. Biosurfactant production by pseudomonas aeruginosa a41. Isolation and biosurfactants production by pseudomonas.
Biosurfactant production by pseudomonas aeruginosa in kefir and fish meal article pdf available in brazilian journal of microbiology 463 july 2015 with 103 reads how we measure reads. The biosurfactant efficiently reduced water surface tension from 72 to 35. Simultaneous production of biopolymer and biosurfactant by. Pseudomonas aeruginosa is a gramnegative bacterium causing nosocomial infections in immunocompromised humans by forming a recalcitrant biofilm, providing resistance to antibiotics 14. In the present study, four species of pseudomonas p.
Biosurfactant production by pseudomonas aeruginosa 181. They include solvent extraction, precipitation and. Biosurfactant production was quantified by st reduction, critical micelle concentration cmc, emulsification capacity ec, and cell surface hydrophobicity csh. According to this results it can be concluded that the biosurfactant is glycolipid nature. In the present investigation, the ability of two bacterial strains bacillus subtilis and pseudomonas aeruginosa to grow on two different carbon sources is presented in table 1. Best results were obtained with olive oil as carbon source. Different kinds of bacteria have been employed by many researchers in producing biosurfactant using. Biodiesel derived waste glycerol as an economic substrate. Biosurfactant production by pseudomonas aeruginosa from. The yield of biosurfactant steadily increased even after a. Multiple roles of biosurfactants in structural biofilm development by. Pseudomonas aeruginosa jbk1 was identified using 16s rrna sequencing. Rhamnolipid biosurfactants produced by pseudomonas species. Rodrigues ibb institute for biotechnology and bioengineering, centre of biological engineering, university of minho, braga, portugal table 1.
20 40 958 1010 1138 1150 65 678 1447 246 1456 9 1163 546 1389 1082 476 365 339 406 580 1108 617 1399 940 1188 301 303 1097 145 1230 454 687 1496 217 503 1093 1154 481 854 852 1197 711 165 311 944 566 102 391 952